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KJO Korean Journal of Orthodontics

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pISSN 2234-7518
eISSN 2005-372X
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Korean J Orthod   

First Published Date September 14, 2022

Copyright © The Korean Association of Orthodontists.

Evaluation of the cell viability and antimicrobial effects of orthodontic bands coated with silver and zinc oxide nanoparticles: an in vitro study

Behrad Tanbakuchi1, Maryam Pourhajibagher2, Alireza Badiei3, Reza Masaeli4, Rashin Bahrami1

1- Department of Orthodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
2-Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3- School of Chemistry, College of Science, University of Tehran, Tehran, Iran
4- Department of Dental Biomaterials, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

Correspondence to:Rashin Bahrami. Orthodontic resident, Department of Orthodontics, School of Dentistry, Tehran University of Medical Sciences, North Kargar St, Tehran, Iran. Tel: +989370053618; Email: bahramirashin@yahoo.com

Abstract

Objective: The present study evaluated the cell viability and antimicrobial effects of orthodontic bands coated with silver and zinc oxide nanoparticles.
Methods: In this experimental study, thirty orthodontic bands were divided into three groups (n=10) of control (uncoated band), silver (Ag) coated band and zinc oxide (ZnO) coated band. The electrostatic spray-assisted vapor deposition method was used to coat silver (Ag) and zinc oxide (ZnO) nanoparticles on orthodontic bands. The biofilm inhibition test assessed the antimicrobial effectiveness of nano-Ag and nano-ZnO against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Biocompatibility tests were carried out using an MTT assay. A One-way ANOVA with a Post-hoc test was used for the comparison between groups.
Results: The reduction in the number of L. acidophilus, C. albicans, and S. mutans colonies around nano-Ag coated bands was significantly higher than around nano-ZnO coated bands (P= 0.015, 0.003, and 0.005, respectively). Compared to the control group, Ag showed a reduction of all microorganisms' ability to replicate at least 2 log10 steps; but ZnO, except for S. mutants, reduced the other two microorganisms to less than 2 log10 steps. The lowest cell viability mean was observed in nano-Ag coated bands, but the difference between the groups was insignificant (P>0.05).
Conclusion: Coating orthodontic bands with ZnO and Ag nanoparticles induced antimicrobial properties against oral pathogens. Among these nanoparticles, the Ag group showed the best antimicrobial activity and the ZnO group showed the highest biocompatibility.

Keywords: Biocompatibility, SEM, Microbiology, Biomaterial science

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Korean J Orthod   

First Published Date September 14, 2022

Copyright © The Korean Association of Orthodontists.

Evaluation of the cell viability and antimicrobial effects of orthodontic bands coated with silver and zinc oxide nanoparticles: an in vitro study

Behrad Tanbakuchi1, Maryam Pourhajibagher2, Alireza Badiei3, Reza Masaeli4, Rashin Bahrami1

1- Department of Orthodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
2-Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3- School of Chemistry, College of Science, University of Tehran, Tehran, Iran
4- Department of Dental Biomaterials, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

Correspondence to:Rashin Bahrami. Orthodontic resident, Department of Orthodontics, School of Dentistry, Tehran University of Medical Sciences, North Kargar St, Tehran, Iran. Tel: +989370053618; Email: bahramirashin@yahoo.com

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: The present study evaluated the cell viability and antimicrobial effects of orthodontic bands coated with silver and zinc oxide nanoparticles.
Methods: In this experimental study, thirty orthodontic bands were divided into three groups (n=10) of control (uncoated band), silver (Ag) coated band and zinc oxide (ZnO) coated band. The electrostatic spray-assisted vapor deposition method was used to coat silver (Ag) and zinc oxide (ZnO) nanoparticles on orthodontic bands. The biofilm inhibition test assessed the antimicrobial effectiveness of nano-Ag and nano-ZnO against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Biocompatibility tests were carried out using an MTT assay. A One-way ANOVA with a Post-hoc test was used for the comparison between groups.
Results: The reduction in the number of L. acidophilus, C. albicans, and S. mutans colonies around nano-Ag coated bands was significantly higher than around nano-ZnO coated bands (P= 0.015, 0.003, and 0.005, respectively). Compared to the control group, Ag showed a reduction of all microorganisms' ability to replicate at least 2 log10 steps; but ZnO, except for S. mutants, reduced the other two microorganisms to less than 2 log10 steps. The lowest cell viability mean was observed in nano-Ag coated bands, but the difference between the groups was insignificant (P>0.05).
Conclusion: Coating orthodontic bands with ZnO and Ag nanoparticles induced antimicrobial properties against oral pathogens. Among these nanoparticles, the Ag group showed the best antimicrobial activity and the ZnO group showed the highest biocompatibility.

Keywords: Biocompatibility, SEM, Microbiology, Biomaterial science